We can provide systems to meet your needs during key areas of oligonucleotide API production, such as synthesis, purification and ultrafiltration.

• In the synthesis stage, we can provide different sizes of synthesizers to meet your needs in different stages of optimization and next step scale-up, from laboratory level to industrial production level;
• In the purification stage, whether you choose reversed-phase chromatography or ion exchange methods, we have the corresponding equipment for you to choose from;
• In the ultrafiltration stage, we can also provide different sizes of equipment to meet your needs for salt exchange concentration.

In a word, with Hanbon, you can find the system that matches your need for your oligonucleotide production process.

Most oligonucleotides are currently synthesized by solid-phase synthesis, using automated equipment, in which phosphoramidite monomers are required. For example, the phosphoramidite monomer for DNA synthesis mainly consists of bases, deoxyribose, 2-cyanoethyl 5'-DMT and 3'-terminus, and diisopropylamine group, while the phosphoramidite monomer for RNA requires protection of 2'-OH to prevent cross-reaction between 2'-OH and 5'-OH in solid-phase synthesis, and the protecting group is usually TBDMS. Solid-phase synthesis is frequently used nowadays, the traditional solid-phase carriers were controlled microporous glass beads (CPG), but the loading capacity was limited, only 70-80 μmol/g. Nowadays, more high-loading capacity solid-phase carriers are used, and the loading capacity is generally up to 250umol/g~350umol/g.