Along with the development of the biopharmaceutical industry, more and more companies are entering into clinical trials and commercial production, and a stable, reproducible and easy-to-use loading process is becoming a demand of more and more companies. Manual column loading faces many problems such as poor reproducibility, time-consuming method development, and complicated maintenance processes.
The theoretical plate number and symmetry factor of a chromatographic column are important indicators of column efficiency. Factors that affect the theoretical column plate number and symmetry factor of chromatography columns include:
In addition, the structural design of the chromatographic column itself also affects the column efficiency of the chromatographic column. Such as the material and surface roughness of the column wall, the tightness of the packing, the uniformity of the mobile phase distribution, etc. Excellent column performance is dependent on the structural design of the column.