The Inline Dilution System is similar to the two-pump gradient mode and allows for buffer dilution within a certain range. However, given the solubility of the mother liquor, pH shift after dilution and other phenomena, the robustness of buffer formulation is limited in large-scale applications.
The in-line conditioning system uses a single component mother liquor of acid system, base system, salt system, water and additive system to perform buffer formulation, simulating the traditional laboratory formulation mode, with corresponding pH and conductivity feedback adjustment added during the formulation process for improving buffer preparation stability.
The in-line buffer configuration is a single-component buffer concentrate with high solubility and can therefore be diluted up to a hundred times, which allows the volume of the buffer tank to be reduced a hundred times and can really solve the efficiency of room utilisation in the dispensing room or between uses.
It is possible to obtain an in-line buffer configuration system that has obvious advantages over in-line dilution systems in terms of scalability and adaptability, and at the same time provides a better solution for modern buffer preparation.
Buffer preparation for chromatography and filtration steps in bioprocessing is a challenge in terms of providing timely buffer delivery, reproducibility, and good process economics.